HiMedia FD025-5X5VL, also known as Vitamino Growth Supplement (Twin Pack), is a chemically defined enrichment used in microbiology to support the growth of a wide variety of microorganisms.
This supplement is particularly important for cultivating nutritionally demanding (fastidious) pathogens, such as Neisseria and Haemophilus species, by providing essential vitamins, amino acids, and coenzymes.
Product Configuration and Packaging
The SKU FD025-5X5VL specifies a bulk packaging format from HiMedia Laboratories.
• Pack Size: 5x5 vials (total of 25 vials per pack).
• Yield: Each individual vial is sufficient for supplementing 500 ml of culture medium, making the entire pack suitable for 12.5 litres of media.
• Twin Pack Format: Each unit consists of two parts: a lyophilized nutrient powder (Part I) and a specific rehydrating fluid (Part II)
Application and Usage
This supplement is typically added to sterile, molten basal media cooled to 45-50°C. It is a standard component for several specialized media:
• GC Agar & Chocolate Agar: Enhances the growth of Neisseria gonorrhoeae and Haemophilus by providing V-factor (NAD) and other nutrients.
• Thayer Martin Medium: Used for the selective isolation of pathogenic Neisseria.
• Tellurite Blood Agar: Used in the cultural response for specific diagnostic protocols
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