Tryptone Soya Broth is a highly nutritious medium used for cultivation of a wide variety of organisms (1). The combination of Tryptone and soya peptone makes the medium nutritious by providing nitrogenous, carbonaceous substances, amino
acids and long chain peptides for the growth of microorganisms. Dextrose/glucose serve as the carbohydrate source and
dibasic potassium phosphate buffer the medium. Sodium chloride maintains the osmotic balance of the medium. Glycerol plays a major role as an anti freezing agent. Hence it is recommended for maintenance of cultures as glycerol serves as an cryopreservant
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