Intended use
Recommended for isolation and differentiation between various species of Bacillus from a mixed culture in foods, clinical and non-clinical samples by chromogenic method.
Composition**
Ingredients g / L
Peptone 10.000
HM extract # 1.000
D-Mannitol 10.000
Sodium chloride 10.000
Chromogenic mixture 3.200
Phenol red 0.025
Agar 15.000
Final pH ( at 25°C) 7.1±0.2
**Formula adjusted, standardized to suit performance parameters
#-Equivalent to Meat extract
Directions
Suspend 49.22 grams in 1000 ml purified /distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121ºC) for 15 minutes. Cool to 45-50°C For selective isolation of B.cereus and B.thuringiensis, aseptically add rehydrated contents of one vial of PB Selective Supplement (FD324). Mix well and pour into sterile Petri plates.
Principle And Interpretation
Majority of Bacillus species apparently have little or no pathogenic potential and are rarely associated with disease in humans or lower animals. The principal exception to this are Bacillus anthracis, the agent of anthrax, and Bacillus cereus, but a number of other species, particularly those of the B.subtilis group, have been implicated in food poisoning and other human and animal infections (1). Bacillus cereus causes food poisoning due to consumption of contaminated rice (2,3,4), other starchy foods such as potato, pasta and cheese have also been implicated, eye infections and a wide range of other clinical conditions like abscess formation, meningitis, septicemia and wound infection.
HiCrome® Bacillus Agar is based on the formulation of MYP Agar formulated by Mossel et al (5) used for enumeration of Bacillus cereus and Bacillus thuringiensis when present in large number in certain foodstuffs.
The medium contains peptone and HM extract, which provide nitrogenous, carbonaceous compounds, long chain amino acids, vitamins and other essential growth nutrients.
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