Intended Use
Recommended for selective isolation of pathogenic Gram positive cocci from various specimens.
Composition
Ingredients g/L
HiVeg® peptone No. 5 20.000
HiVeg®™ infusion 3.000
Corn starch 1.000
Sodium chloride 5.000
Colistin sulphate 0.010
Nalidixic acid 0.015
Agar 15.000
Final pH (at 25°C) 7.3±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 44.02 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 5% v/v sterile, defibrinated blood. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Columbia Agar Base is a nutritionally rich formula containing 5% defibrinated blood, which provides more nutrients and capability of displaying haemolytic reactions. Columbia Blood Agar Base is utilized as a base for preparation of media containing blood and in selective media preparations where various combinations of antimicrobial agents are used as additives. Ellner et al formulated the medium (1) and found the combination of peptones used gave more rapid and abundant growth of Streptococci, Staphylococci, Neisseria and Haemophilus with better-defined haemolytic reactions. Columbia C.N.A. HiVeg®™ Agar Base is prepared with the same formula as Columbia Agar Base with the addition of 10 mg/litre of colistin and 15 mg/litre of nalidixic acid to inhibit the growth of gram-negative bacteria and to support the growth of Staphylococci, haemolytic Streptococci and Enterococci when supplemented with 5% blood.
Columbia C.N.A. HiVeg® Agar Base is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones. HiVeg®™ peptone No. 5 and HiVeg®™ infusion supports luxuriant growth of microorganisms and visualization of good haemolytic reactions. Sheep blood allows detection of haemolytic reactions and supplies
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