HiMedia M2089, also known as Burkholderia Cepacia Selective Agar (BCSA), is a specialized culture medium used for the selective isolation and identification of Burkholderia cepacia from clinical samples (such as respiratory secretions of cystic fibrosis patients) and pharmaceutical products.
Composition and Specifications
According to the HiMedia Laboratories technical data sheet, the M2089 formulation includes the following key ingredients (in g/L):
• Casitose (Casein Peptone): 10.000 (Nitrogen and nutrient source)
• Lactose & Sucrose: 10.000 each (Fermentable carbohydrates)
• Yeast Extract: 1.500 (Vitamin B complex source)
• Sodium Chloride: 5.000 (Maintains osmotic balance)
• Selective Agents: Crystal violet (0.002), Gentamicin (0.010), Vancomycin (0.0025), and Polymyxin B (600,000 units).
• pH Indicator: Phenol red (0.080)
• Final pH: 7.0 ± 0.1 at 25°C
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